You are here: Experiment Guide > Overview of AFM Operation > Align Laser

Align the Laser

  1. To align the laser on the AFM probe, click the Setup button in the workflow toolbar to open the Setup view.
  1. New users will probably find the laser alignment procedure easier by clicking Alignment Station icon. This will move the stage so that the Dimension Icon head is positioned over the Alignment Station mirror and select the Tip Reflection option in the Focus Select panel.
CAUTION: Do not use the Alignment Station when using the High Efficiency Direct Drive TappingMode Fluid Probe Holder as it will hit the Alignment Station and knock it off its magnetically secured base. If you have selected a fluid experiment, the Alignment station button will be grayed out.

Laser Alignment

HINT: To see the laser spot, you may need to turn down the illuminator intensity before proceeding with laser alignment.

For both TappingMode AFM and Contact AFM, the user aligns the laser by moving the laser beam relative to the cantilever while observing the laser spot on the granite surface (a piece of white paper also works well) below the Dimension Icon SPM Scanner. If the laser is not on the cantilever or probe substrate, the laser appears as a bright red spot on the surface below. When the laser is aligned on the cantilever, a shadow appears on the surface below.

The laser control knobs are located on the top of the Dimension Icon scanner. There is a diagram printed on top of the Dimension Icon scanner illustrating which direction the laser moves when turning the laser control knobs clockwise:

The right-rear laser control knob, atop the Dimension Icon scanner, controls the laser beam movement along the X direction—along the major axis of the substrate (parallel to the length of the cantilever).

The front-left laser knob, atop the scanner, moves the beam along the Y direction perpendicular to the cantilever and substrate's major axis. In the vision system display, the X direction is right-to-left across the screen, and the Y direction is top-to-bottom.

The procedure for aligning the laser is slightly different depending on the Probe Tip Geometry. The sections below detail the procedures for aligning the laser on the cantilever and tips for each mode respectively.

Etched Silicon Tips (TappingMode)
Silicon Nitride Tips (Contact Mode AFM)

Previous Steps:

  1. Select Experiment
  2. Prepare and Load the Probe Holder
  3. Install the Probe Holder on the SPM Scanner
  4. Identify the Probe

Next Steps:

  1. Adjust the Photodetector
  2. Locate the Tip
  3. Load the sample and Focus on the Sample Surface
  4. If your experiment involves TappingMode, you must now Tune the Cantilever before proceeding to the next step. If you are running a Contact Mode application, continue to Check Initial Scan Parameters.
  5. Engage, Scan, & Withdraw

 

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